Engineering the Caenorhabditis elegans genome with CRISPR/Cas9
1Department of Biology, Utrecht University, 3584 CH Utrecht, The Netherlands.
Methods (San Diego, Calif.)
|April 2, 2014
Summary
CRISPR/Cas9 genome engineering allows precise DNA modification in model organisms. This review details its adaptation for Caenorhabditis elegans, offering guidelines for its use in generating mutations and specific genomic changes.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- CRISPR/Cas9 technology emerged in 2013 for advanced genome engineering.
- This system utilizes a guide RNA and Cas9 endonuclease to target specific DNA sequences.
- The Cas9 enzyme induces double-strand breaks, enabling gene mutation or precise editing via homologous recombination.
Purpose of the Study:
- To review the adaptation of CRISPR/Cas9 for the model organism Caenorhabditis elegans.
- To provide practical guidelines for implementing CRISPR/Cas9-based genome engineering in C. elegans.
Main Methods:
- Adaptation of CRISPR/Cas9 for C. elegans genome editing.
- Utilizing guide RNA to direct Cas9 endonuclease activity.
- Employing imprecise repair for mutations and homologous recombination for specific edits.
Main Results:
- Successful application of CRISPR/Cas9 in C. elegans for generating mutations.
- Demonstrated ability to create tailored genome modifications through homologous recombination.
- Development of distinct strategies for CRISPR/Cas9 implementation in C. elegans.
Conclusions:
- CRISPR/Cas9 technology offers powerful tools for C. elegans genome engineering.
- The reviewed approaches facilitate precise genetic manipulation in this model system.
- Guidelines are provided to aid researchers in CRISPR/Cas9-based genome engineering in C. elegans.
Related Concept Videos
01:28CRISPR/Cas9 Genome Editing
3.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.2K
01:59CRISPR
46.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.4K
01:59CRISPR
13.9K
13.9K
02:53CRISPR and crRNAs
14.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.6K