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Human monocyte activation induced by an anti-CD14 monoclonal antibody
1Department of Medical Immunology, Ernst-Moritz-Arndt-University, Greifswald, G.D.R.
Immunology Letters
|December 1, 1988
Abstract:
An anti-CD14 mAb RoMo-1 rapidly induces in human monocytes a transient oxidative burst activity as detected by chemiluminescence assay. Pretreatment of these cells with the mAb markedly suppresses the monocyte chemiluminescence response to opsonized zymosan. In addition, the antibody induces a significant increase of IL-1 production and secretion by mononuclear cells, comparable to a similar effect of rIFN-gamma or LPS. Electron microscopy demonstrates internalization of the CD14 molecules after interaction with the mAb in a characteristic receptor-like manner.