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Ultraviolet A inhibits histamine release from human peripheral leukocytes
J Ring1, B Przybilla, B Eberlein
1Dermatologische Klinik und Poliklinik, Ludwig-Maximilians-Universität München, FRG.
International Archives of Allergy and Applied Immunology
|January 1, 1989
Summary
Ultraviolet A (UVA) irradiation significantly reduces histamine release from human leukocytes, an effect mediated by free radicals. This finding holds true for both atopic and non-atopic individuals.
Area of Science:
- Immunology
- Photobiology
Background:
- Leukocytes play a crucial role in allergic responses, including histamine release.
- Ultraviolet radiation is known to have immunomodulatory effects.
Purpose of the Study:
- To investigate the effect of ultraviolet A (UVA) irradiation on histamine release from human peripheral leukocytes.
- To determine the role of free radicals in UVA-induced modulation of histamine release.
Main Methods:
- Human peripheral leukocytes were irradiated with varying doses of UVA.
- Histamine release was stimulated using anti-IgE, Ca-ionophore, and C5a.
- The effect of superoxide dismutase, a radical scavenger, was assessed.
Main Results:
- UVA irradiation caused a dose-dependent reduction in anti-IgE and Ca-ionophore-stimulated histamine release.
- C5a-induced histamine release was not consistently affected by UVA.
- Superoxide dismutase abolished the inhibitory effect of UVA on anti-IgE-stimulated histamine release.
- Ultraviolet B (UVB) exposure did not significantly alter in vitro histamine release.
Conclusions:
- UVA irradiation inhibits specific pathways of histamine release from human leukocytes.
- The inhibitory effect of UVA is mediated by free radicals.
- These findings suggest a potential role for UVA in modulating allergic inflammatory responses.