Related Experiment Video
Updated: May 1, 2026
12:26
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
Published on: June 2, 2018
7.2K
A step-wise approach to deep brain stimulation in mice
Casey H Halpern1, Mark A Attiah, Anand Tekriwal
1Department of Neurosurgery, University of Pennsylvania, 3400 Spruce St., Philadelphia, PA, 19104, USA, casey.halpern@uphs.upenn.edu.
Acta Neurochirurgica
|April 2, 2014
Summary
Deep brain stimulation (DBS) is now feasible in mice, overcoming size and handling challenges. This breakthrough enables studying neurological disorders in mice, advancing research for human treatments.
Area of Science:
- Neuroscience
- Behavioral Science
- Medical Engineering
Background:
- Deep brain stimulation (DBS) research in mice is limited due to species-specific challenges.
- Murine models are crucial for understanding human behavior and neurological disorders.
- Overcoming these challenges is vital for advancing DBS research.
Purpose of the Study:
- To establish a safe and effective stereotactic deep brain electrode implantation technique in mice.
- To investigate the feasibility of DBS studies in a murine model.
- To pave the way for using mice in DBS research for neuropsychiatric disorders.
Main Methods:
- Stereotactic electrode implantation using mechanical skull etching and dental acrylate fixation.
- Stimulation experiments conducted in the home cage post-habituation.
- Post-experiment verification of electrode placement in fixed tissue.
Main Results:
- Safe and accurate electrode implantation in mice for DBS experimentation was achieved.
- High accuracy in electrode placement within the nucleus accumbens shell was demonstrated.
- No hardware malfunctions occurred during experimentation, ensuring procedural integrity.
Conclusions:
- Stereotactic DBS studies can be safely and effectively performed in mice.
- This technique facilitates the investigation of neuropsychiatric disorders in murine models.
- Widely available transgenic mouse lines and the Cre-Lox system can enhance mechanistic studies.
