Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-wavelength holographic micro-endoscopy.

Optics express·2024
Same author

Lensless imaging in one shot using the complex degree of coherence obtained by multiaperture interferences.

Optics letters·2024
Same author

Lensless microscopy by multiplane recordings: sub-micrometer, diffraction-limited, wide field-of-view imaging.

Optics express·2023
Same author

Advantages of holographic imaging through fog.

Applied optics·2023
Same author

Physics-enhanced neural network for phase retrieval from two diffraction patterns.

Optics express·2022
Same author

Intrinsic parameter-free calibration of FPP using a ray phase mapping model.

Optics letters·2022

Related Experiment Video

Updated: May 1, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

9.5K

Opposed-view dark-field digital holographic microscopy.

Ahmad Faridian1, Giancarlo Pedrini1, Wolfgang Osten1

  • 1Institut für Technische Optik and Stuttgart Research Center of Photonic Engineering (SCoPE), University of Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany.

Biomedical Optics Express
|April 2, 2014
PubMed
Summary
This summary is machine-generated.

This study introduces an opposed-view dark-field digital holographic microscope (DHM) to overcome scattering and absorption issues in biological imaging. The novel DHM system enhances internal structure contrast and signal-to-noise ratio for clearer specimen visualization.

Keywords:
(090.1995) Digital holography(100.2980) Image enhancement(180.3170) Interference microscopy(180.6900) Three-dimensional microscopy

More Related Videos

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

11.5K
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Published on: November 1, 2017

9.8K

Related Experiment Videos

Last Updated: May 1, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

9.5K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

11.5K
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Published on: November 1, 2017

9.8K

Area of Science:

  • Biomedical Imaging
  • Microscopy Techniques
  • Optical Physics

Background:

  • Scattering and absorption hinder deep-layer imaging in biological specimens.
  • Structural inhomogeneity causes varying light interception and spatial frequencies from different depths.
  • Conventional microscopy struggles with contrast and signal-to-noise ratio for internal structures.

Purpose of the Study:

  • To develop an advanced imaging method to improve contrast and signal-to-noise ratio (SNR) in biological specimens.
  • To address challenges posed by light scattering and absorption in internal layer imaging.
  • To enable multilayer imaging without physical depth scanning.

Main Methods:

  • Utilized an opposed-view dark-field digital holographic microscope (DHM).
  • Concurrently collected scattered light from both top and bottom views of the specimen.
  • Implemented digital refocusing for multilayer image reconstruction.

Main Results:

  • Achieved enhanced contrast of internal biological structures.
  • Significantly improved the signal-to-noise ratio in the acquired images.
  • Demonstrated successful multilayer imaging of a Drosophila embryo using the DHM system.

Conclusions:

  • The opposed-view DHM effectively mitigates scattering and absorption issues in biological imaging.
  • This technique offers a robust solution for high-contrast, high-SNR imaging of internal specimen layers.
  • Digital holographic microscopy provides a powerful platform for advanced biological sample analysis.