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High TMEM45A expression is correlated to epidermal keratinization
Aurélie Hayez1, Jérémy Malaisse, Edith Roegiers
1Cell and Tissue Laboratory-URPhyM-NARILIS, University of Namur, Namur, Belgium.
Experimental Dermatology
|April 3, 2014
Summary
The transmembrane protein 45A (TMEM45A) gene is highly expressed in skin cells and its protein accumulates during epidermal keratinization, suggesting a key role in this vital skin process.
Area of Science:
- Cell Biology
- Dermatology
- Molecular Biology
Background:
- The TMEM45A gene encodes a transmembrane protein highly expressed in epidermal keratinocytes.
- Its precise role in skin differentiation and keratinization remains largely uncharacterized.
Purpose of the Study:
- To investigate the involvement of TMEM45A in epidermal differentiation and keratinization.
- To determine the subcellular localization of TMEM45A in keratinocytes.
Main Methods:
- Characterization of TMEM45A expression in normal human keratinocytes in vitro and in vivo.
- Subcellular localization studies using co-detection with ER and Golgi markers.
- Analysis of TMEM45A mRNA and protein in epidermal and thymic tissues.
Main Results:
- TMEM45A expression is upregulated during keratinocyte differentiation induced by confluence or increased calcium.
- The protein is primarily localized to the granular layer of the epidermis in vivo.
- TMEM45A associates with the Golgi apparatus, specifically the trans-Golgi/trans-Golgi network.
Conclusions:
- TMEM45A expression is strongly correlated with epidermal keratinization.
- The gene plays a significant role in the keratinization process.
- TMEM45A is not associated with lysosomes or lamellar bodies in keratinocytes.
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