Related Experiment Video
Updated: May 1, 2026
08:07
The Microfluidic Probe: Operation and Use for Localized Surface Processing
Published on: June 4, 2009
8.4K
A compact and versatile microfluidic probe for local processing of tissue sections and biological specimens
J F Cors1, R D Lovchik1, E Delamarche1
1IBM Research-Zurich, Saeumerstrasse 4, CH-8803 Rueschlikon, Switzerland.
The Review of Scientific Instruments
|April 3, 2014
Summary
A compact microfluidic probe (cMFP) enables precise, non-contact local processing of biological surfaces. This user-friendly tool enhances tissue analysis and cellular interactions in research and diagnostics.
Area of Science:
- Biotechnology
- Microfluidics
- Surface Science
Background:
- Microfluidic probes (MFPs) offer non-contact, localized biochemical processing of surfaces.
- Existing MFPs require specialized setups, limiting their widespread adoption.
Purpose of the Study:
- To develop a compact microfluidic probe (cMFP) for integration with standard inverted microscopes.
- To enhance the functionality and user-friendliness of microfluidic probes for biological applications.
Main Methods:
- The cMFP system was designed with a small footprint and scanning capabilities (45 × 45 mm²) with ±15 μm accuracy.
- Self-aligned MFP heads, rapid liquid switching (90 s), and height-compensation for non-planar surfaces were implemented.
- Demonstrated local processing on tissue sections (melanoma cell blocks) and copper-coated topographies.
Main Results:
- The cMFP successfully performed local staining on tissue sections, creating various patterns like contours, lines, and gradients.
- Height-compensation enabled patterning on surfaces with up to 750 μm height differences.
- The system demonstrated ease of use with joystick control and scripting capabilities.
Conclusions:
- The compact, user-friendly cMFP is suitable for standard life science laboratories.
- This technology facilitates precise interaction with tissues and cells for research, development, and diagnostics.
