Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial transcriptome for cell segmentation.

Nature computational science·2026
Same author

Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026
Same author

Binocular vision fusion enhanced 3D NIR-II <i>in vivo</i> imaging of bone and vessel networks.

Fundamental research·2026
Same author

Doppler-enhanced displacement measurement method based on scanning reference gratings.

Optics express·2026
Same author

Beads-on-a-Tip testing for ultrasensitive antigen detection across a large dynamic range.

Smart molecules : open access·2026

Related Experiment Video

Updated: May 1, 2026

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

11.5K

Schlieren confocal microscopy for phase-relief imaging.

Hao Xie, Dayong Jin, Junjie Yu

    Optics Letters
    |April 3, 2014
    PubMed
    Summary

    We developed a phase-sensitive microscopy technique using Schlieren modulation and confocal laser scanning microscopy (CLSM) to image transparent specimens. This method quantifies phase gradients and integrates with existing systems for multimodal imaging.

    More Related Videos

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.4K
    Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera
    07:22

    Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

    Published on: May 6, 2016

    6.9K

    Related Experiment Videos

    Last Updated: May 1, 2026

    Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
    10:56

    Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

    Published on: May 20, 2014

    11.5K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.4K
    Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera
    07:22

    Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

    Published on: May 6, 2016

    6.9K

    Area of Science:

    • Optical Microscopy
    • Biophysics
    • Image Analysis

    Background:

    • Transparent specimens lack contrast in standard microscopy.
    • Phase gradients in specimens cause light refraction, which is difficult to image directly.
    • Confocal laser scanning microscopy (CLSM) offers high resolution but typically images fluorescence.

    Purpose of the Study:

    • To develop a simple phase-sensitive microscopic technique.
    • To image the phase gradient of transparent specimens.
    • To enable multimodal fluorescence and structure imaging within a single system.

    Main Methods:

    • Utilized Schlieren modulation combined with confocal laser scanning microscopy (CLSM).
    • Employed a secondary illumination created by specimen-induced refraction exciting a fluorescence plate.
    • Integrated a partial obstructor before the confocal pinhole to modulate fluorescence.

    Main Results:

    • Demonstrated a technique capable of imaging the phase gradient of transparent specimens.
    • Established a quantitative relationship between image intensity and sample phase gradient.
    • Showcased the adaptability of the setup to current confocal systems.

    Conclusions:

    • The developed phase-sensitive microscopy technique is simple and effective.
    • The method allows for quantitative imaging of phase gradients in transparent samples.
    • This approach facilitates simultaneous acquisition of fluorescence and structural information.