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Updated: May 1, 2026

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
Published on: August 12, 2013
Implementation of a Gaussian beam laser and aspheric optics for high spatial resolution MALDI imaging MS
Andre Zavalin1, Junhai Yang, Andreas Haase
1Mass Spectrometry Research Center, National Research Resource for Imaging Mass Spectrometry, Vanderbilt University, Nashville, TN, USA.
Abstract:
We have investigated the use of a Gaussian beam laser for MALDI Imaging Mass Spectrometry to provide a precisely defined laser spot of 5 μm diameter on target using a commercial MALDI TOF instrument originally designed to produce a 20 μm diameter laser beam spot at its smallest setting. A Gaussian beam laser was installed in the instrument in combination with an aspheric focusing lens. This ion source produced sharp ion images at 5 μm spatial resolution with signals of high intensity as shown for images from thin tissue sections of mouse brain.

