Flash photolysis of caged compounds: new tools for cellular physiology

Caged compounds are molecules or ions of physiological interest, e.g. ATP, IP3, cAMP, cGMP, GTP and Ca2+ rendered inactive by chemical modification. The modification introduces a photochemically labile bond, which on exposure to ultraviolet light cleaves rapidly, releasing the active compound. This article reviews some of the major advances and applications of the photorelease approach, and illustrates its potential in several areas of interest to cellular neuroscientists.