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Related Concept Videos

Patch Clamp01:18

Patch Clamp

6.0K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.0K

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Updated: May 1, 2026

One-channel Cell-attached Patch-clamp Recording
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Patch-clamp recording of voltage-sensitive Ca2+ channels.

María A Gandini1, Alejandro Sandoval, Ricardo Felix

  • 1Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, Mexico;

Cold Spring Harbor Protocols
|April 3, 2014
PubMed
Summary

The patch-clamp technique refines voltage-clamp methods for measuring ionic currents. This study highlights its application in studying voltage-gated calcium (CaV) channels and their molecular physiology.

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Area of Science:

  • Biophysics
  • Molecular Physiology
  • Cell Biology

Background:

  • Traditional voltage-clamp methods measure currents from cells or membrane areas.
  • The patch-clamp technique is a refinement offering single-channel and whole-cell recording capabilities.
  • The whole-cell configuration is the most common method for recording ionic currents.

Purpose of the Study:

  • To focus on the patch-clamp technique as a refinement of voltage-clamp methods.
  • To detail the application of patch-clamp in studying voltage-gated (CaV) Ca(2+) channels.
  • To discuss aspects of the molecular physiology of CaV channels.

Main Methods:

  • Patch-clamp technique (whole-cell configuration).
  • Voltage-clamp electrophysiology.
  • Analysis of ionic currents.

Main Results:

  • The patch-clamp technique enables recording of ionic currents through single channels.
  • Whole-cell patch-clamp is widely used for ionic current recordings.
  • The technique is particularly useful for studying voltage-gated Ca(2+) channels.

Conclusions:

  • The patch-clamp technique significantly advanced electrophysiological recordings.
  • Its application provides insights into the function of voltage-gated Ca(2+) channels.
  • Further understanding of CaV channel molecular physiology is facilitated by this method.