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Generating size-controlled embryoid bodies using laser direct-write.

A D Dias1, A M Unser, Y Xie

  • 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, NY 12180, USA.

Biofabrication
|April 4, 2014
PubMed
Summary
This summary is machine-generated.

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Laser direct-write printing controls embryoid body size by adjusting cell density. This method offers precise control over stem cell aggregates for future differentiation studies.

Area of Science:

  • Stem Cell Biology
  • Biotechnology
  • Regenerative Medicine

Background:

  • Embryonic stem cells (ESCs) can self-renew and differentiate into specialized cell types.
  • Embryoid bodies (EBs) are 3D aggregates used for in vitro ESC differentiation, influenced by size.
  • Previous studies show both EB and 2D colony size impact differentiation.

Purpose of the Study:

  • To investigate if laser direct-write (LDW) printing can control mouse ESC (mESC) embryoid body size.
  • To determine the independent effects of printed colony size and cell density on EB formation.
  • To establish a method for engineering EB size for directed differentiation.

Main Methods:

  • Mouse ESCs were printed using LDW at varying colony sizes and densities.
  • Embryoid body diameter was measured after three days.

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  • Two-way ANOVAs and Tukey's honestly significant difference tests were used for statistical analysis.

    • Main Results:

    • EB diameter was significantly influenced by printing density (p = 0.0002) but not printed colony size (p = 0.74).
    • High-density printed colonies resulted in significantly larger EBs.
    • LDW demonstrated independent control over local cell density and colony size.

    Conclusions:

    • EB size can be engineered by controlling the printing density of mESCs using LDW.
    • LDW printing offers precise spatial control for stem cell aggregation and differentiation.
    • This technique has potential for improved stem cell maintenance and directed differentiation protocols.