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Streptococcus suis serotyping by a new multiplex PCR.

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Summary

A new multiplex PCR assay effectively identifies numerous Streptococcus suis serotypes in both human and pig samples. This method aids in the surveillance of Streptococcus suis, a significant pathogen in veterinary and public health.

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Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Molecular Diagnostics

Background:

  • Streptococcus suis is a significant zoonotic pathogen causing meningitis and other infections in pigs and humans.
  • Accurate and efficient serotyping is crucial for epidemiological surveillance and control of Streptococcus suis outbreaks.
  • Existing methods for Streptococcus suis serotyping can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop and validate a multiplex Polymerase Chain Reaction (PCR) assay for the simultaneous detection of multiple Streptococcus suis serotypes.
  • To assess the assay's performance on both human and swine isolates.
  • To provide a tool for improved serotype surveillance of Streptococcus suis.

Main Methods:

  • Development of a multiplex PCR assay comprising four reaction sets targeting various Streptococcus suis serotypes.
  • Testing the assay with reference strains and clinical isolates from human and pig sources.
  • Evaluation of specificity by challenging the assay with other bacterial species.

Main Results:

  • The multiplex PCR assay successfully identified multiple Streptococcus suis serotypes, including 1/2, 1, 2, 3, 7, 9, 11, 14, 16, 4, 5, 8, 12, 18, 19, 24, 25, 6, 10, 13, 15, 17, 23, 31, 21, 27, 28, 29, and 30.
  • The assay accurately detected serotypes 2, 5, 14, and 24 in human isolates.
  • The assay correctly identified a broad range of serotypes (1-17, 19, 24, 28, 31) in pig isolates from Thailand.
  • No cross-reactivity was observed with other bacterial species, indicating high specificity.

Conclusions:

  • The developed multiplex PCR assay is a reliable and efficient tool for the simultaneous detection of a wide range of Streptococcus suis serotypes.
  • This assay is valuable for routine serotyping and epidemiological surveillance of Streptococcus suis in both human and animal populations.
  • The assay's ability to detect multiple serotypes in a single reaction offers a significant advantage over traditional methods.