Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Destabilization of Microtubules

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction: The KrasG12D;Trp53fl/fl murine model of undifferentiated pleomorphic sarcoma is macrophage dense, lymphocyte poor, and resistant to immune checkpoint blockade.

PloS one·2026

Same author

Novel Pattern of Nuclear Staining With ROS1 Immunohistochemistry: A Case Report.

Case reports in pulmonology·2026

Same author

Neutrophil extracellular traps offer a new therapeutic target for elephant endotheliotropic herpes virus-hemorrhagic disease (EEHV-HD).

Communications biology·2026

Same author

Rapid activation of ARF6 after RAF inhibition augments BRAF<sup>V600E</sup> and promotes therapy resistance.

Oncogene·2026

Same author

Impact of Surgical Margins in Chest Wall Chondrosarcomas: A CanSaRCC Study.

Journal of surgical oncology·2026

Same author

Elective Surgical Care Pathways Are Associated With Lower Fracture Rates at Presentation and Contribute to Superior Clinical Outcomes in Metastatic Bone Disease.

Journal of surgical oncology·2026

Same journal

Tissue MicroRNAs in Arrhythmogenic Cardiomyopathy: A Systematic Review of Studies in Human Myocardium and Animal Models with Implications for Post-Mortem Molecular Diagnostics.

Genes·2026

Same journal

Genetic Variants and Dental Caries Susceptibility: An Umbrella Review and Multilevel Meta-Analysis.

Genes·2026

Same journal

Generative AI and Language Models in Human Genetics and Health: From Variant Interpretation to Clinical Decision Support.

Genes·2026

Same journal

Familial White-Sutton Syndrome Caused by a Pathogenic POGZ p.Arg508* Variant: Intrafamilial Variability from Childhood to Adulthood.

Genes·2026

Same journal

Genetic Influence on LDL-Cholesterol Levels: Role of Polygenic Risk Scores and Lp(a) Beyond Monogenic Hypercholesterolemia.

Genes·2026

Same journal

THBS1 as a Key Regulator of Myoblasts: Validation of Its Inhibitory Roles in Skeletal Muscle Development.

Genes·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 1, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

Microsatellites with macro-influence in ewing sarcoma.

Michael J Monument¹, Kirsten M Johnson², Allie H Grossmann³

¹Center for Children's Cancer Research, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA. Michael.Monument@hci.utah.edu.

|April 8, 2014

Summary

This summary is machine-generated.

GGAA microsatellites, once considered junk DNA, are crucial for EWS/FLI gene regulation in Ewing sarcoma. Their polymorphic nature impacts cancer susceptibility and prognosis, offering new insights into oncogenesis.

More Related Videos

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

Published on: December 9, 2016

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

Related Experiment Videos

Last Updated: May 1, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

Published on: December 9, 2016

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Chromosomal translocations create fusion proteins driving tumorigenesis.
Ewing sarcoma involves EWS/ETS fusions, notably EWS/FLI, which are potent oncoproteins.
EWS/FLI drives oncogenesis through a unique transcriptional signature.

Purpose of the Study:

Investigate the role of GGAA microsatellites in EWS/FLI-mediated gene upregulation.
Explore the functional significance of GGAA microsatellites in Ewing sarcoma pathogenesis.
Understand how EWS/ETS fusions influence cancer susceptibility and prognosis via microsatellites.

Main Methods:

Analysis of EWS/FLI binding to GGAA microsatellite elements.
Assessment of microsatellite influence on gene expression.
Examination of GGAA microsatellite polymorphism in different populations.

Main Results:

EWS/FLI upregulates gene expression via GGAA microsatellite response elements.
These microsatellites act as enhancers and are sites of epigenetic regulation.
Increased GGAA motifs enhance EWS/FLI activity, with population-specific variations.

Conclusions:

Microsatellite DNA, specifically GGAA repeats, plays a critical role in Ewing sarcoma oncogenesis.
GGAA microsatellites are essential for EWS/FLI transcriptional regulation and oncogenic target upregulation.
The polymorphic nature of these microsatellites offers insights into cancer susceptibility and prognosis.