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Basic techniques for studies of iNKT cells and MAIT cells.

Asako Chiba1, Sachiko Miyake

  • 1Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|April 8, 2014
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Summary

Invariant natural killer T (iNKT) cells and mucosal-associated invariant T cells (MAIT cells) are innate-like lymphocytes. This study details methods for preparing, detecting, and analyzing these crucial immune cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • T cell subsets

Background:

  • Invariant natural killer T (iNKT) cells and mucosal-associated invariant T cells (MAIT cells) are innate-like lymphocytes.
  • These cells express semi-invariant T cell receptors and perform diverse immune functions.
  • Their abundance in human peripheral blood suggests significant physiological roles.

Purpose of the Study:

  • To describe cell preparation techniques for innate-like lymphocytes.
  • To introduce methods for detecting and functionally analyzing iNKT cells and MAIT cells.

Main Methods:

  • Cell preparation techniques for innate-like lymphocytes.
  • Detection methods for iNKT and MAIT cells.
  • Functional analysis protocols for iNKT and MAIT cells.

Main Results:

  • Established protocols for isolating and preparing innate-like lymphocytes.
  • Detailed methods for identifying and quantifying iNKT and MAIT cells.
  • Introduced approaches for assessing the diverse functions of these T cell subsets.

Conclusions:

  • Provides essential methodologies for studying iNKT and MAIT cells.
  • Facilitates further research into the physiological roles of these innate-like lymphocytes.
  • Aims to advance understanding of immune responses involving iNKT and MAIT cells.