Related Experiment Video

Updated: May 1, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.9K

Transient state imaging of live cells using single plane illumination and arbitrary duty cycle excitation pulse

Jonas Mücksch1, Thiemo Spielmann1, Evangelos Sisamakis1

  • 1Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, Albanova University Center, 106 91, Stockholm, Sweden.

Journal of Biophotonics
|April 8, 2014
PubMed

Keywords:
fluorescencelive cell imagingredox statesingle plane illumination microscopytransient state imagingtriplet state

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K
Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage CoTrAC11:31

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage CoTrAC

Published on: March 15, 2013

9.3K

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K