Anti-inflammatory labdane diterpenoids from Lagopsis supina

Area of Science:

• Natural Product Chemistry
• Pharmacology

Background:

• Lagopsis supina is a plant source of bioactive compounds.
• Labdane diterpenoids are a class of natural products with diverse biological activities.

Purpose of the Study:

• To isolate and characterize new labdane diterpenoids from Lagopsis supina.
• To evaluate the inhibitory effects of isolated compounds on nitric oxide production in microglial cells.

Main Methods:

• Isolation and purification of compounds using chromatographic techniques.
• Structure elucidation by spectroscopic methods, including single-crystal X-ray crystallography.
• In vitro assay for nitric oxide inhibition in lipopolysaccharide-stimulated BV-2 microglial cells.

Main Results:

• Ten new labdane diterpenoids, lagopsins A-H and 15-epi-lagopsins C and D, were identified.
• Five known labdane diterpenoids were also isolated.
• Compounds 7, 9, 13, and 15 demonstrated moderate inhibition of nitric oxide production (IC50: 14.9–34.9 μM).

Conclusions:

• Lagopsis supina is a rich source of novel labdane diterpenoids.
• Some isolated compounds possess anti-inflammatory potential by inhibiting nitric oxide production in microglial cells.