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Optimization of medium composition for exopolysaccharide production by Phellinus nigricans
Zhanyong Wang1, Yue Quan1, Feng Zhou1
1College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Carbohydrate Polymers
|April 9, 2014
Summary
Optimized Phellinus nigricans culture medium significantly boosted exopolysaccharide (EPS) production by 2.3-fold. The resulting P. nigricans exopolysaccharide (PNEP) demonstrated potent antioxidant and radical scavenging properties.
Area of Science:
- Mycology
- Biotechnology
- Biochemistry
Background:
- Phellinus nigricans is a fungus known for producing bioactive compounds.
- Exopolysaccharides (EPS) are complex carbohydrates with diverse biological activities.
- Optimizing culture media is crucial for maximizing microbial metabolite yields.
Purpose of the Study:
- To optimize the culture medium for Phellinus nigricans to maximize exopolysaccharide production.
- To characterize the antioxidant properties of the produced exopolysaccharide.
Main Methods:
- Sequential statistical optimization using Plackett-Burman design, steepest ascent method, and Box-Behnken design.
- Determination of optimal concentrations for glucose, peptone, yeast extract, thiamine, KH2PO4, and MgSO4.
- Assays for radical scavenging activity (superoxide anion, DPPH, hydroxyl) and reducing power.
Main Results:
- Optimized medium composition: 51.67g/L glucose, 6.96g/L peptone, 1.0g/L yeast extract, 5mg/L thiamine, 1.0g/L KH2PO4, and 0.5g/L MgSO4.
- Achieved exopolysaccharide yield of 1.89±0.13g/L, a 2.3-fold increase compared to unoptimized medium.
- The exopolysaccharide (PNEP) consisted of glucose and mannose (1:2.63 molar ratio) and exhibited significant antioxidant and radical scavenging activities.
Conclusions:
- The statistical optimization strategy effectively enhanced Phellinus nigricans exopolysaccharide production.
- The optimized PNEP possesses notable antioxidant properties, suggesting potential applications.
- Further research into PNEP's structure-activity relationship and applications is warranted.
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