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Automated quantification of rat plasma acute phase reactants in experimental inflammation.
E J Lewis1, J Bishop, C H Cashin
1Department of Biology, Roche Products Limited, Welwyn Garden City, Hertfordshire, England.
Journal of Pharmacological Methods
|May 1, 1989
Summary
This study quantifies acute-phase reactants (APRs) in rat plasma during arthritis. Drug treatments showed varied effects on APR profiles, indicating distinct mechanisms of anti-inflammatory action.
Area of Science:
- Biochemistry
- Pharmacology
- Immunology
Background:
- Acute-phase reactants (APRs) are key biomarkers in inflammation.
- Quantifying APRs aids in understanding inflammatory disease progression and drug efficacy.
Purpose of the Study:
- To quantify specific acute-phase reactants (albumin, iron, fibrinogen, seromucoid, haptoglobin, ceruloplasmin) in rat plasma.
- To investigate the effects of anti-inflammatory drugs on APR profiles during adjuvant-induced arthritis.
Main Methods:
- Utilized the COBAS-BIO centrifugal analyzer for plasma quantification.
- Induced arthritis in rats and monitored APR levels over 5 days.
- Administered anti-inflammatory drugs (indomethacin, dexamethasone, clobuzarit) and assessed their impact on APRs and edema.
Main Results:
- APR levels correlated with the degree of inflammation in a dose-dependent manner.
- Anti-inflammatory drugs reduced edema but exhibited differential effects on APR profiles.
- Specific drug classes demonstrated defined impacts on acute-phase protein concentrations.
Conclusions:
- Multiple APR analysis can help elucidate mechanisms of action for anti-inflammatory and antirheumatic drugs.
- APR profiling offers a systemic measure to differentiate drug effects in inflammatory diseases.