Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal Testing of Exploratory Behaviour in Mice Reveals Stable Cognitive Traits Across the Adult Lifespan.

Aging cell·2025
Same author

Survey of healthcare-associated sink infrastructure, and sink trap antibiotic residues and biochemistry, in twenty-nine UK hospitals.

The Journal of hospital infection·2025
Same author

[Correlation analysis between expression of cytokeratin 19 and clinical pathological characteristics and prognosis of dual-phenotype hepatocellular carcinoma].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2025
Same author

[CK19 is associated with poor survival in patients with dual-phenotype hepatocellular carcinoma].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024
Same author

[Clinical, imaging, and pathological characteristics of 35 cases of mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes syndrome].

Zhonghua nei ke za zhi·2024
Same author

A workflow for the detection of antibiotic residues, measurement of water chemistry and preservation of hospital sink drain samples for metagenomic sequencing.

The Journal of hospital infection·2023
Same journal

Combined genome and transcriptome analysis of boll weight and lint percentage traits in Gossypium barbadense.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

The allelic variation of anthocyanidin reductase underlies anthocyanin biosynthesis and purple leaf trait in Brassica napus.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Unveiling core genomic regions shaping plant architecture, productivity, and seed quality traits in sesame (Sesamum indicum L.): insights from Meta-QTL study into breeding targets.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Watkins wheat landraces: a treasure of stripe rust resistance alleles identified using multi-model association analyses.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Selection of four mutant alleles of fatty acid desaturase genes for a stable high oleic and low linolenic acid soybean seed oil trait.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Harnessing artificial intelligence in plant breeding: innovations in digital phenotyping and breeding methodologies.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

11.2K

A simple PCR-based method for scoring the ph1b deletion in wheat.

L J Qu1, T N Foote, M A Roberts

  • 1Cereals Research Department, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK, GB.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|April 9, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed a new PCR-based assay to detect the Ph1 gene deletion in wheat. This method is faster, more accurate, and automatable compared to traditional techniques for wheat genetics.

More Related Videos

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues
07:17

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

1.8K
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

11.4K

Related Experiment Videos

Last Updated: May 1, 2026

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

11.2K
Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues
07:17

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

1.8K
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

11.4K

Area of Science:

  • Plant genetics
  • Molecular biology
  • Wheat breeding

Background:

  • The Ph1 gene in wheat (Triticum aestivum) is crucial for regulating homologous chromosome pairing during meiosis.
  • Mutations or deletions in the Ph1 gene, such as in the Chinese Spring ph1b mutant, lead to homoeologous chromosome pairing.
  • Accurate identification of Ph1 gene status is important for wheat genetic studies and breeding.

Purpose of the Study:

  • To develop a rapid and reliable molecular marker for the Ph1 gene deletion in wheat.
  • To provide an alternative to laborious and less accurate cytological methods for screening Ph1 gene status.
  • To facilitate large-scale screening of wheat populations for Ph1 gene deletion.

Main Methods:

  • Amplified Fragment Length Polymorphism (AFLP) was employed to identify unique DNA sequences in euploid wheat Chinese Spring compared to the ph1b mutant.
  • Polymorphic DNA fragments were cloned, sequenced, and utilized to design specific PCR primers.
  • A PCR-based assay was established using these primers to differentiate between the presence and absence of the Ph1 gene deletion.
  • The assay was validated on a doubled-haploid population segregating for the ph1b deletion.

Main Results:

  • Specific DNA sequences absent in the ph1b mutant but present in euploid Chinese Spring were identified using AFLP.
  • Two primer pairs were designed based on these sequences, enabling specific amplification via PCR.
  • The PCR assay successfully distinguished between wheat lines with and without the Ph1 gene deletion.
  • The assay demonstrated high reliability and accuracy when tested on a segregating population.
  • The PCR assay can be performed directly on alkaline-treated wheat leaves, simplifying sample preparation.

Conclusions:

  • A novel, efficient, and automatable PCR-based screening method for the Ph1 gene deletion in wheat has been developed.
  • This molecular assay offers significant advantages in terms of speed, labor, accuracy, and reliability over conventional cytological methods.
  • The developed assay is a valuable tool for wheat genetic research and breeding programs requiring precise screening of Ph1 gene status.