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Updated: May 1, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Synthesis of 3,3'-di-O-methyl ardimerin and exploration of its DNA binding properties
Miran Mavlan1, Kevin Ng, Harmanpreet Panesar
1Department of Chemistry and Biochemistry, California State University , Northridge 18111 Nordhoff Street, Northridge, California 91330, United States.
Abstract:
The 3,3'-di-O-methyl derivative (15) of the bis-C-aryl glycoside natural product ardimerin (1) has been synthesized in 11 steps from 2,3,4,6-tetrabenzylglucose (2) and 1,2,3-trimethoxybenzene (3). Key steps in the synthesis involve a Lewis acid mediated Friedel-Crafts type glycosylation and a Yamaguchi lactonization under Yonemitsu conditions. 3,3'-Di-O-methyl ardimerin aggregates in aqueous solutions at concentrations greater than 1 μM, and both UV and fluorescence binding studies indicate that 15 has a low affinity for duplex DNA.

