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Related Experiment Video

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Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
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Testing a prototype pulse generator for a continuous flow system and its use for E. coli inactivation and microalgae

Karel Flisar1, Sasa Haberl Meglic1, Jernej Morelj2

  • 1University of Ljubljana, Faculty of Electrical Engineering, Laboratory of Biocybernetics, Trzaska 25, 1000 Ljubljana, Slovenia.

Bioelectrochemistry (Amsterdam, Netherlands)
|April 10, 2014
PubMed
Summary

Continuous pulsed electric field (PEF) treatment effectively inactivates E. coli and extracts microalgae lipids. This flow system offers a promising alternative to traditional methods for pathogen inactivation and bioprocessing.

Keywords:
Continuous flow systemEscherichia coli inactivationMicroalgae lipid extractionPEF

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Area of Science:

  • Biotechnology
  • Microbiology
  • Bioprocess Engineering

Background:

  • Electroporation is utilized for pathogen inactivation and substance extraction from microorganisms.
  • Large-scale flow systems are increasingly employed in these applications.
  • Pulsed Electric Fields (PEF) offer a non-thermal method for cell membrane permeabilization.

Purpose of the Study:

  • To evaluate a continuous flow pulse generator for Pulsed Electric Field (PEF) treatment.
  • To assess the efficacy of PEF for Escherichia coli inactivation in a flow system.
  • To investigate the application of continuous flow PEF for microalgae lipid extraction.

Main Methods:

  • A continuous flow system was developed for PEF treatment.
  • Flow rate was controlled to ensure a defined number of pulses per bacterial cell.
  • Lipid extraction from Chlorella vulgaris was performed using the PEF flow system and compared to conventional methods.

Main Results:

  • PEF flow treatment demonstrated effective E. coli inactivation, comparable to batch cuvette systems.
  • The number of pulses directly correlated with the extent of bacterial inactivation.
  • Continuous flow electroporation directly ruptured microalgae cells in broth, allowing for lipid skimming.
  • An initial oil yield of 50% was achieved compared to organic solvent extraction.

Conclusions:

  • Continuous flow PEF is a viable method for large-scale E. coli inactivation.
  • PEF offers an efficient, solvent-free approach for microalgae lipid extraction.
  • This technology streamlines bioprocessing by bypassing multiple conventional extraction stages.