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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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The dynamic epitranscriptome: N6-methyladenosine and gene expression control.

Kate D Meyer1, Samie R Jaffrey1

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Nature Reviews. Molecular Cell Biology
|April 10, 2014
PubMed
Summary
This summary is machine-generated.

N(6)-methyladenosine (m(6)A) is a widespread mRNA modification, forming the epitranscriptome. This discovery reveals new pathways for post-transcriptional gene regulation by m(6)A writers, erasers, and readers.

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Area of Science:

  • Molecular Biology
  • RNA Biology
  • Epigenetics

Background:

  • N(6)-methyladenosine (m(6)A) is a known RNA modification.
  • Previous understanding of m(6)A prevalence was limited.
  • m(6)A is analogous to DNA methylation in regulating gene function.

Purpose of the Study:

  • To redefine the understanding of m(6)A prevalence across the transcriptome.
  • To explore the role of m(6)A in post-transcriptional mRNA regulation.
  • To investigate the components of the m(6)A epitranscriptome.

Main Methods:

  • Transcriptome-wide m(6)A mapping studies.
  • Identification of m(6)A 'writers' (methyltransferases).
  • Identification of m(6)A 'erasers' (demethylating enzymes).
  • Identification of m(6)A 'readers' (binding proteins).

Main Results:

  • m(6)A modification is present in a substantial subset of the transcriptome.
  • m(6)A occurs in specific regions of mRNA.
  • The pattern of m(6)A constitutes an mRNA epitranscriptome.

Conclusions:

  • m(6)A is a widespread mRNA modification with significant regulatory potential.
  • The discovery of m(6)A writers, erasers, and readers defines cellular pathways for mRNA regulation.
  • The m(6)A epitranscriptome offers new insights into post-transcriptional gene control.