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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Programmable pH-triggered DNA nanoswitches.

Andrea Idili¹, Alexis Vallée-Bélisle, Francesco Ricci

¹Dipartimento di Scienze e Tecnologie Chimiche, University of Rome, Tor Vergata , 00133, Rome, Italy.

Journal of the American Chemical Society

|April 11, 2014

Summary

Programmable DNA nanoswitches can be precisely tuned to open or close at specific pH levels. This breakthrough enables the development of advanced pH nanosensors for real-time monitoring in various applications.

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Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

DNA nanotechnology offers versatile platforms for creating responsive materials.
pH-triggered molecular switches are crucial for biological and chemical sensing.
Existing pH sensors often lack tunable sensitivity and broad dynamic range.

Purpose of the Study:

To design and characterize programmable DNA-based nanoswitches.
To demonstrate tunable pH-dependent switching behavior.
To develop a broad-range pH nanosensor using these DNA switches.

Main Methods:

Constructed intramolecular triplex DNA structures utilizing pH-sensitive Hoogsteen interactions.
Modified the TAT/CGC triplet content to rationally tune pH sensitivity.
Integrated multiple nanoswitches with distinct pH sensitivities for multi-point sensing.

Main Results:

Achieved tunable pH triggering across more than 5 pH units by altering DNA sequence composition.
Demonstrated a novel pH nanosensor capable of monitoring pH variations over a 5.5 pH unit range.
Observed fast response times (<200 ms) and high reversibility in the DNA nanoswitches.

Conclusions:

Programmable DNA nanoswitches with tunable pH dependence have been successfully engineered.
These nanoswitches enable the creation of highly sensitive and broadly responsive pH nanosensors.
The developed technology holds significant potential for in vivo pH monitoring and smart nanomaterials.