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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
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Outer membrane protein OmpW is the receptor for typing phage VP5 in the Vibrio cholerae O1 El Tor biotype

Donglei Xu1, Jingyun Zhang1, Jie Liu1

  • 1State Key Laboratory for Infectious Disease Prevention and Control and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.

Journal of Virology
|April 11, 2014
PubMed

Insights

Outer membrane protein OmpW is the receptor for Vibrio cholerae phage VP5. An 11-bp deletion in the ompW gene of resistant strains can serve as a marker for cholera surveillance.

Area of Science:

  • Microbiology
  • Bacteriology
  • Virology

Background:

  • Phage typing is a method for subtyping bacterial clones, particularly useful for tracking epidemic strains.
  • Vibrio cholerae O1 El Tor biotype is a significant cause of cholera outbreaks.
  • Phage resistance can arise through mutations affecting bacterial surface receptors.

Purpose of the Study:

  • To identify the specific bacterial receptor for the phage VP5, a key tool in Vibrio cholerae O1 El Tor biotype typing.
  • To investigate the genetic basis of VP5 resistance in epidemic Vibrio cholerae strains.
  • To evaluate the potential of identified genetic markers for use in public health surveillance.

Main Methods:

  • Phage susceptibility testing of Vibrio cholerae isolates.
  • Identification and characterization of bacterial outer membrane proteins.
  • Genetic sequencing to identify mutations in receptor genes.
  • Comparative genomic analysis of phage-resistant and phage-sensitive strains.

Main Results:

  • The outer membrane protein OmpW was identified as the specific receptor for phage VP5.
  • A consistent 11-base pair deletion in the ompW gene was found in all epidemic strains resistant to phage VP5.
  • This ompW deletion was absent in VP5-sensitive strains, indicating its role in resistance.

Conclusions:

  • OmpW is the primary receptor for phage VP5 in Vibrio cholerae O1 El Tor.
  • The 11-bp deletion in ompW represents a specific genetic marker for VP5 resistance.
  • This marker holds potential for tracing epidemic Vibrio cholerae strains and enhancing cholera surveillance efforts.

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