A model to induce scattered cell damage in the corneal endothelium

A model to induce scattered cell damage in the corneal endothelium was set up by shaking a pig cornea in a glass bottle with 0.18% NaCl. Disseminated endothelial-cell losses, whose amounts correlated with the durations of treatment, were observed in the corneas which were shaken for less than 8 min. Following organ culturing for 1 week, most of the corneas still possessed intact endothelia which means that even after the treatment the endothelium still keeps a normal regenerative ability. With this method of design, a good model is provided to study the regeneration of the corneal endothelium.