Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.4K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
3.4K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

5.8K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
5.8K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

3.0K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
3.0K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

3.3K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
3.3K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

5.9K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is...
5.9K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrogen Bonding in Graphene.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Multimodal Nanoscale Mapping of Local Structure and CO<sub>2</sub> Adsorption in Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same author

Quantitative model for infrared nano-spectroscopy in liquid.

Optics express·2025
Same author

Graphene-Gated Control of Ag Nanowire Infrared Polaritons.

Nano letters·2025
Same author

Correlated nanoimaging of structure and dynamics of cation-polaron coupling in hybrid perovskites.

Science advances·2025
Same author

Ti<sub>3</sub>C<sub>2</sub>T <sub></sub> MXene Thin Films and Intercalated Species Characterized by IR-to-UV Broadband Ellipsometry.

The journal of physical chemistry. C, Nanomaterials and interfaces·2025

Related Experiment Video

Updated: May 1, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.2K

Vibrational nano-spectroscopic imaging correlating structure with intermolecular coupling and dynamics.

Benjamin Pollard1, Eric A Muller1, Karsten Hinrichs2

  • 11] Department of Physics, Department of Chemistry, and JILA, University of Colorado, Boulder, Colorado 80309, USA [2].

Nature Communications
|April 12, 2014
PubMed
Summary

This study introduces a new microscopy technique to visualize nanoscale molecular interactions. This method precisely maps molecular environments, enabling better control over material properties for applications like organic solar cells.

More Related Videos

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

8.4K
Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

8.2K

Related Experiment Videos

Last Updated: May 1, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.2K
Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

8.4K
Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

8.2K

Area of Science:

  • Materials Science
  • Spectroscopy
  • Nanotechnology

Background:

  • Nanoscale molecular interactions are crucial for self-assembly, biomembranes, and organic solar cells.
  • Current imaging techniques lack the resolution, sensitivity, or specificity to fully understand these interactions.
  • Controlling nanoscale properties requires advanced imaging of molecular behavior.

Purpose of the Study:

  • To develop and implement a high-resolution optical microscopy technique for nanoscale molecular analysis.
  • To investigate structure-function relationships in nano-phase separated block copolymers.
  • To correlate molecular-scale morphology with local chemical environments and electric fields.

Main Methods:

  • Vibrational scattering-scanning near-field optical microscopy (VS-SNOM) with high spectral precision.
  • Utilizing vibrational resonance as a reporter for local chemical environments.
  • Imaging solvatochromic Stark shifts and line broadening at nanometre resolution.

Main Results:

  • Achieved few nanometre spatial resolution and 0.2 cm(-1) spectral precision.
  • Directly imaged nanoscale morphology and correlated it with intermolecular interactions.
  • Quantitatively discriminated local electric field variations between nano-domains.

Conclusions:

  • The developed VS-SNOM technique provides direct insight into nanoscale morphology and molecular interactions.
  • This capability facilitates systematic control over the functionality of multicomponent soft matter systems.
  • Enables advancements in materials science, particularly for organic electronics and biomimetic systems.