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[Nucleolar apparatus function and the ribosomal gene activity of mouse embryonic fibroblasts infected by the SA7(C8)
Abstract:
Computer morphometry revealed statistically significant (P less than 0.001) decrease in the number and area of nucleoli and ribosomal gene activities (as demonstrated by AgNOR staining) in primary mouse embryonal fibroblast cultures infected by oncogenic adenovirus SA7(C8). Even more drastic increased in these parameters was found when serum-depleted cultures were infected. There were no effects on nucleolar and AgNOR parameters in mock-infected cultures. It is suggested that an increase in the ribosomal gene activity may reflect synthesis of SA7(C8) early gene products in target cells.
Insights
Oncogenic adenovirus SA7(C8) infection significantly decreased nucleoli and ribosomal gene activity in mouse cells. This effect was more pronounced in serum-depleted cells, suggesting viral early gene product synthesis.
Area of Science:
- Molecular Biology
- Cell Biology
- Virology
Background:
- Adenoviruses are common viruses that can cause illness.
- Oncogenic viruses have the potential to cause cancer.
- Nucleoli and ribosomal gene activity are crucial for cell growth and function.
Purpose of the Study:
- To investigate the effect of oncogenic adenovirus SA7(C8) on nucleolar and ribosomal gene activity in mouse embryonal fibroblast cultures.
- To determine if serum depletion influences the virus's impact on these cellular parameters.
Main Methods:
- Computer morphometry was used to analyze nucleolar and ribosomal gene activity.
- AgNOR staining was employed to assess ribosomal gene activity.
- Primary mouse embryonal fibroblast cultures were infected with adenovirus SA7(C8) or mock-infected.
- Cultures were either normally supplemented or serum-depleted.
Main Results:
- A statistically significant decrease (P < 0.001) in nucleoli number and area, as well as ribosomal gene activity, was observed in adenovirus-infected cells.
- Serum-depleted cultures exhibited a more drastic reduction in these parameters following infection.
- Mock-infected cultures showed no changes in nucleolar or AgNOR parameters.
Conclusions:
- Oncogenic adenovirus SA7(C8) infection significantly impacts nucleolar structure and ribosomal gene expression in mouse fibroblasts.
- The observed decrease in ribosomal gene activity may be linked to the synthesis of early gene products of the SA7(C8) adenovirus.
- Serum depletion exacerbates the effects of adenovirus infection on cellular ribosomal activity.