Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

13.7K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OTUB1 non-canonically inhibits TAB2 ubiquitination to govern microglia-mediated neuroinflammation.

EMBO molecular medicine·2026
Same author

The calcium pump ATP2B1/PMCA1 regulates CNS vascular development by facilitating Norrin- and WNT7A/B-induced Frizzled4 signaling.

Cell reports·2026
Same author

Emerging concepts, hot topics, and open questions in today's pancreatic cancer research.

Biochimica et biophysica acta. Reviews on cancer·2026
Same author

Immune-metabolic trajectories delineate subgroups in paediatric long COVID.

Nature communications·2026
Same author

Prospective genomic and epidemiologic surveillance of Klebsiella pneumoniae in a tertiary NICU.

Antimicrobial resistance and infection control·2026
Same author

Interferon-Regulatory Factor 4 Is Required Not Only for Induction but Also for Maintenance of the Th17 Phenotype.

European journal of immunology·2026
Same journal

Fast Generation of F(Ab')<sub>2</sub> Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme.

European journal of immunology·2026
Same journal

Generation of Regulatory T Cells Against Islet Neoantigen.

European journal of immunology·2026
Same journal

Complement Inhibition in the Clinic: Are We Doing Enough to Protect Patients From Infection?

European journal of immunology·2026
Same journal

Special Issue: yEFIS 3rd Symposium.

European journal of immunology·2026
Same journal

CRISPR/Cas9-Mediated Gene Knockout Reveals a Nonredundant Role for p16<sup>INK4A</sup> in Controlling TCR-Dependent and Independent CD8 T Cell Expansion.

European journal of immunology·2026
Same journal

Induction of Humoral and Cellular Immunity After SARS-CoV-2 JN.1 Vaccination in Individuals With and Without Prior Infection.

European journal of immunology·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Isolation and Th17 Differentiation of Na&#239;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

33.7K

CTLA-4 (CD152) enhances the Tc17 differentiation program.

Jonas Pick1, Aditya Arra, Holger Lingel

  • 1Department of Pediatrics, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany.

European Journal of Immunology
|April 12, 2014
PubMed
Summary
This summary is machine-generated.

Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) promotes IL-17-producing CD8(+) T cells (Tc17 cells), impairing their ability to clear infections. Inactivating CTLA-4 may enhance host defense against bacterial pathogens.

Keywords:
CTLCostimulationIFN-γIL-17IpilimumabT-cell differentiation

More Related Videos

Isolation, Cultivation, and Transient Transfection of Primary Human T Cells to Generate Chimeric Antigen Receptor (CAR) T Cells
08:26

Isolation, Cultivation, and Transient Transfection of Primary Human T Cells to Generate Chimeric Antigen Receptor (CAR) T Cells

Published on: March 27, 2026

326
Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

3.2K

Related Experiment Videos

Last Updated: May 1, 2026

Isolation and Th17 Differentiation of Na&#239;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

33.7K
Isolation, Cultivation, and Transient Transfection of Primary Human T Cells to Generate Chimeric Antigen Receptor (CAR) T Cells
08:26

Isolation, Cultivation, and Transient Transfection of Primary Human T Cells to Generate Chimeric Antigen Receptor (CAR) T Cells

Published on: March 27, 2026

326
Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

3.2K

Area of Science:

  • Immunology
  • Cellular Biology
  • Infectious Disease

Background:

  • CD8(+) T cells (Tc17 cells) producing IL-17 are crucial for host defense but exhibit diminished cytotoxic activity.
  • Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a key regulator of T cell responses.
  • The specific role of CTLA-4 in Tc17 cell differentiation and function remains incompletely understood.

Purpose of the Study:

  • To investigate the role of CTLA-4 in the differentiation and function of IL-17-producing CD8(+) T cells (Tc17 cells).
  • To determine if CTLA-4 influences the cytotoxic capacity and cytokine production profile of Tc17 cells.
  • To evaluate the therapeutic potential of targeting CTLA-4 for enhancing host defense against intracellular bacterial infections.

Main Methods:

  • Coculture systems involving CTLA-4(+/+) and CTLA-4(-/-) T cells.
  • Agonistic CTLA-4 stimulation.
  • In vivo infection models using Listeria monocytogenes (LM).
  • Analysis of cytokine production (IL-17, IFN-γ, TNF-α) and cytotoxic molecule expression (granzyme B).
  • Assessment of Tc17-related gene expression (IL-17A, RORγt, IRF-4).

Main Results:

  • CTLA-4 was found to intrinsically enhance the frequency of IL-17 production in CD8(+) T cells, promoting Tc17 differentiation.
  • CTLA-4(-/-) Tc17 cells exhibited enhanced bacterial clearance capacity compared to wild-type Tc17 cells in a Listeria monocytogenes infection model.
  • Adoptively transferred CTLA-4(-/-) Tc17 cells expressed granzyme B and produced Tc1 cytokines (IFN-γ, TNF-α) upon rechallenge.
  • CTLA-4 signaling upregulated key Tc17-related molecules, including IL-17A, RORγt, and IRF-4, confirming a robust Tc17 differentiation program.

Conclusions:

  • CTLA-4 promotes Tc17 cell differentiation, leading to robust Tc17 responses that may compromise host defense against intracellular pathogens.
  • Inactivation of CTLA-4 enhances the quality of Tc17 cells, restoring cytotoxic activity and promoting a mixed Tc1/Tc17 cytokine profile.
  • Targeting CTLA-4 represents a potential therapeutic strategy to improve the clearance of intracellular bacterial infections by enhancing CD8(+) T cell-mediated immunity.