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Updated: May 1, 2026
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
A simple ex vivo model for teaching Descemet membrane endothelial keratoplasty
Konstantinos Droutsas1, Michael Petrak, Gerrit R J Melles
1Department of Ophthalmology, Philipps University, Marburg, Germany; First Department of Ophthalmology, Medical School of Athens, University of Athens, Athens, Greece; The Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands.
Purpose:
To describe a simple ex vivo porcine model for teaching Descemet Membrane Endothelial Keratoplasty (DMEK).
Methods:
Twenty freshly enucleated porcine eyes were used to create a pseudo-graft from the porcine lens capsule and implant it into an intact porcine globe using the standardized 'no-touch' technique of the Netherlands Institute for Innovative Ocular Surgery (NIIOS).
Results:
The DMEK pseudo-graft could be inserted, centred, unfolded and appositioned in all cases.
Conclusions:
Practicing DMEK surgery under wetlab conditions was feasible by the described simple and low-cost model. This new tool may facilitate the adaptation of DMEK by cornea surgeons.
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