Plastic operations of burns using cultured epithelium

Autoradiographic and electron-microscopic methods were used for the study of cultures of human skin epidermocytes in media with a low calcium content. It has been demonstrated that the culturing of epidermocytes in a medium containing 0.15 mM of calcium suppresses differentiation and stimulates division of cells. The considerable number of dividing cells in the cultures permits their use both for grafting on wound surfaces and further cultivation.