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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Bioplasmonic calligraphy for multiplexed label-free biodetection.

Limei Tian¹, Sirimuvva Tadepalli¹, Sang Hyun Park¹

¹Department of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St. Louis, St Louis, MO 63130, USA.

Biosensors & Bioelectronics

|April 15, 2014

Summary

Plasmonic calligraphy uses gold nanorods as ink in a pen to create sensitive, multiplexed bioassays on paper. This biofriendly method overcomes limitations of conventional printing for point-of-care diagnostics.

Keywords:

Calligraphy Gold nanorods Localized surface plasmon resonance Plasmonic ink

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Area of Science:

Biomedical Engineering
Nanotechnology
Biosensing

Background:

Developing printable multi-marker biochips for simultaneous detection is crucial for point-of-care diagnostics.
Preserving biomolecule functionality during conventional printing remains a significant challenge.

Purpose of the Study:

To introduce plasmonic calligraphy for creating multiplexed label-free bioassays.
To demonstrate a biofriendly and efficient method for fabricating sensitive biosensors.

Main Methods:

Utilizing a ballpoint pen filled with biofunctionalized gold nanorods as plasmonic ink.
Creating isolated test domains on paper substrates for miniaturized biosensor fabrication.
Employing a label-free detection strategy.

Main Results:

Plasmonic calligraphy enables the creation of few mm(2) test domains, significantly improving plasmonic biosensor sensitivity.
The method facilitates multiplexed biodetection and the development of multi-marker biochips on a single test strip.
This approach offers an alternative to conventional inkjet printing, with potential for automation.

Conclusions:

Plasmonic calligraphy is a simple, powerful, and biofriendly technique for fabricating advanced biodiagnostic tools.
The method enhances biosensor sensitivity and enables multiplexed detection, crucial for resource-limited settings.
This approach represents a significant advancement in printable biodiagnostics and biochip development.