Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

46.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.4K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.2K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytochrome P450 1a (CYP1A)-Knockout Javanese Medaka Fish Exhibit Diabetic Traits and Reduced Reproductive Capacity.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka.

Development, growth & differentiation·2026
Same author

Influence of melanocortin-4 receptor (mc4r) genome editing on taste and odor components of red sea bream Pagrus major.

Bioscience, biotechnology, and biochemistry·2026
Same author

Revised taxonomy reveals sustained introgression and secondary contact in ancient lake ricefishes.

BMC ecology and evolution·2026
Same author

A Simple Method for RNA-Seq of Manually Isolated Chromatophores in Oryzias Fishes.

Development, growth & differentiation·2026
Same author

A genetic model of congenital intestinal atresia implicates Mypt1 in epithelial organisation.

Disease models & mechanisms·2026

Related Experiment Video

Updated: May 1, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

24.0K

Targeted mutagenesis using CRISPR/Cas system in medaka.

Satoshi Ansai1, Masato Kinoshita2

  • 1Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.

Biology Open
|April 15, 2014
PubMed
Summary

CRISPR/Cas9 gene editing was successfully used for targeted mutagenesis in medaka fish. This RNA-guided endonuclease (RGEN) system efficiently induced mutations in the DJ-1 gene, showing promise for genome editing applications.

Keywords:
CRISPR/CasGenome editingMedakaMutagenesisOff-target alterations

More Related Videos

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.2K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.7K

Related Experiment Videos

Last Updated: May 1, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

24.0K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.2K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Aquaculture

Background:

  • The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system offers a powerful method for targeted genome editing.
  • RNA-guided endonucleases (RGENs) derived from CRISPR/Cas systems provide a versatile platform for precise DNA modification.
  • Medaka fish (Oryzias latipes) are a valuable model organism for genetic studies in aquatic species.

Purpose of the Study:

  • To demonstrate the efficacy of CRISPR/Cas-mediated RGENs for targeted mutagenesis in medaka embryos.
  • To investigate the efficiency of inducing both somatic and heritable mutations at a specific gene locus.
  • To assess potential off-target effects and explore strategies for minimizing them.

Main Methods:

  • Medaka embryos were injected with single-guide RNA (sgRNA) transcribed by a T7 promoter and Cas9 nuclease mRNA.
  • Targeted mutagenesis was assessed at the DJ-1 gene locus.
  • Off-target analysis was performed on genomic loci with sequence similarity to the target site.

Main Results:

  • High efficiency of targeted mutagenesis was achieved at the DJ-1 gene, resulting in both somatic and heritable mutations.
  • sgRNAs targeting sequences adjacent to NGG protospacer adjacent motif (PAM) sequences were effective, indicating broad applicability.
  • Off-target alterations were observed at loci with double mismatches but could be reduced by lowering sgRNA dosage.

Conclusions:

  • CRISPR/Cas-mediated RGENs are an efficient and flexible tool for genome editing in medaka.
  • The system allows for precise induction of mutations, facilitating genetic manipulation in this important model organism.
  • Optimizing sgRNA dosage can mitigate off-target effects, enhancing the safety and reliability of CRISPR/Cas editing in medaka.