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Related Experiment Video

Updated: Jun 12, 2026

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

Published on: October 6, 2022

Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka.

Miwa Kuroyanagi1, Satoshi Ansai2, Keigo Okamoto3,4

  • 1Department of Advanced Aquaculture Science, Faculty of Marine Bioscience and Technology, Fukui Prefectural University, Obama, Fukui, Japan.

Development, Growth & Differentiation
|June 10, 2026
PubMed
Summary

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This summary is machine-generated.

A standardized protocol for assessing Japanese medaka sperm motility using computer-aided sperm motility analysis (CASA) ensures reproducible results. Early measurements post-activation are crucial for accurate data, especially when evaluating cryopreserved sperm.

Area of Science:

  • Reproductive Biology
  • Aquatic Toxicology
  • Environmental Risk Assessment

Background:

  • Sperm motility is a key indicator of reproductive health, sensitive to environmental factors.
  • Inconsistent measurements hinder accurate assessment of sperm motility.
  • Standardization is needed for reliable sperm motility analysis in research.

Purpose of the Study:

  • To develop a standardized protocol for assessing Japanese medaka sperm motility.
  • To evaluate the impact of cryopreservation on sperm motility.
  • To establish optimal timing for post-activation motility measurements.

Main Methods:

  • Utilized a sperm motility analysis system (SMAS) with computer-aided sperm motility analysis (CASA).
  • Detailed procedures for sperm collection, activation, and quantitative motility assessment.
Keywords:
computer‐aided sperm motility analysis (CASA)medakasperm motilitysperm motility analysis system (SMAS)

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Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)
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Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Published on: December 11, 2020

Related Experiment Videos

Last Updated: Jun 12, 2026

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

Published on: October 6, 2022

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)
05:31

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Published on: December 11, 2020

  • Investigated time-dependent changes and cryopreservation effects on motility.

    • Main Results:

    • Demonstrated time-dependent declines in sperm motility and velocity post-activation.
    • Cryopreservation accelerated motility decline but did not affect initial motile sperm proportion.
    • Early measurements (within 1 minute) are critical for capturing peak motility.

    Conclusions:

    • The standardized SMAS-based protocol offers a robust framework for medaka sperm motility analysis.
    • Early post-activation measurements are essential for consistent and reliable data.
    • This protocol supports research in reproductive biology, toxicology, and aquacultural breeding.