Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K
The Proteasome Structure01:17

The Proteasome Structure

2.2K
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
2.2K
The Proteasome02:18

The Proteasome

3.9K
3.9K
The Proteasome01:13

The Proteasome

1.6K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.6K
The Proteasome02:18

The Proteasome

7.8K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
7.8K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PROTOCOL: Utilisation of Genetics and Genomics in Primary Care: Protocol for an Evidence and Gap Map.

Campbell systematic reviews·2026
Same author

Reproducible and sensitive detection of simple and complex glycan isomers using a new porous graphitized carbon phase packed in micro-bore geometries.

Talanta·2026
Same author

Integrative multiomic analysis reveals co-ordinated alternative splicing in human bone marrow stromal stem cells.

Scientific reports·2026
Same author

Offline Two-Stage SEC and LC-MS/MS for Comprehensive Characterization of Yeast Ribosomal Populations.

Journal of proteome research·2026
Same author

Correction to: Lipopolysaccharide and Morphine-3-Glucuronide-Induced Immune Signalling Increases the Expression of Polysialic Acid in PC12 Cells (PMID- 31646464).

Molecular neurobiology·2026
Same author

Modulation of glycosylation in the brain: A single-cell exploration of glycosidases using the Allen Brain Cell Atlas.

Biochimica et biophysica acta. General subjects·2026
Same journal

Proteome-level evidence that tebuconazole, both alone and in interaction with thiacloprid, affects epigenetic events in bumblebee heads.

Journal of proteomics·2026
Same journal

Proteomics-based analysis of the defense mechanisms of disease-resistant grass carp against Aeromonas veronii.

Journal of proteomics·2026
Same journal

On-filter fractionation by empFASP improves identification of membrane peptides in proteomic experiments.

Journal of proteomics·2026
Same journal

Transpulmonary proteomic gradient analysis in women with pulmonary arterial hypertension associated with systemic sclerosis.

Journal of proteomics·2026
Same journal

Multiomic insights into fungal polylactic acid degradation: Metabolic adaptation and hydrolytic mechanisms of Sporobolomyces pararoseus.

Journal of proteomics·2026
Same journal

Temporal proteomic analysis reveals a three-phase adaptation strategy in Phytophthora cinnamomi during salinity stress.

Journal of proteomics·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.4K

A Sydney proteome story.

Keith L Williams1, Andrew A Gooley2, Marc R Wilkins3

  • 1BKG Group, 2B Mosman St, Mosman, NSW 2088, Australia.

Journal of Proteomics
|April 17, 2014
PubMed
Summary
This summary is machine-generated.

This article chronicles a multidisciplinary team's journey through the emergence of proteomics, detailing their transition from academia to industry. Their work significantly advanced large-scale protein analysis, transforming protein science.

Keywords:
2-D gelsAutomationMass spectrometryProteome history

More Related Videos

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

1.4K
Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
10:12

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Published on: October 28, 2021

3.3K

Related Experiment Videos

Last Updated: May 1, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.4K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

1.4K
Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
10:12

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Published on: October 28, 2021

3.3K

Area of Science:

  • Proteomics and Protein Science
  • Biotechnology and Scientific Instrumentation

Background:

  • The emergence of proteomics revolutionized biological sciences by enabling large-scale protein analysis.
  • The narrative focuses on the experiences of a Macquarie University-based multidisciplinary group.

Observation:

  • The group transitioned from conventional academic research to establishing a Major National Research Facility.
  • This period saw significant advancements and challenges in developing new technologies for protein analysis.

Findings:

  • The team's efforts, alongside global collaborators, contributed to the transformation of protein science.
  • The development of large-scale protein analysis capabilities marked a paradigm shift in biological research.

Implications:

  • The field of proteomics has fundamentally altered biological research methodologies and understanding.
  • The journey highlights the interplay between academic research, technological innovation, and commercialization in scientific advancement.