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Updated: May 1, 2026

Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization
Published on: May 18, 2018
Hyperlactemia induction modes affect the lactate minimum power and physiological responses in cycling
Alessandro M Zagatto1, Johnny Padulo, Paulo T G Müller
11Department of Physical Education, Sao Paulo State University-UNESP, Bauru, Brazil; 2Faculty of Medicine and Surgery, University of "Tor Vergata," Rome, Italy; 3Tunisian Research Laboratory "Sports Performance Optimization" National Center of Medicine and Science in Sport, Tunis, Tunisia; 4Laboratory of Research in Exercise Physiology, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Brazil; and 5School of Physical Education and Sport of Ribeirão Preto, University of Sao Paulo (USP), Ribeirão Preto, Brazil.
The method used to induce hyperlactemia (high blood lactate) and acidosis significantly impacts lactate minimum intensity (LMI) and physiological markers in athletes. Different exercise protocols alter blood pH and lactate levels, influencing LMI results.
Area of Science:
- Exercise Physiology
- Sports Science
- Biochemistry
Background:
- Lactate minimum intensity (LMI) is a crucial physiological marker in endurance athletes.
- Understanding factors influencing LMI, such as hyperlactemia and acidosis, is vital for performance optimization.
- Previous research has not fully elucidated the impact of different induction methods on LMI and associated physiological responses.
Purpose of the Study:
- To investigate the influence of varying hyperlactemia and blood acidosis induction methods on lactate minimum intensity (LMI).
- To compare physiological parameters at LMI across different exercise protocols in trained cyclists and triathletes.
Main Methods:
- Twenty recreationally trained male cyclists and triathletes performed three lactate minimum tests.
- Exercise protocols included a graded exercise test (GXT), a Wingate test (WAnT), and two consecutive Wingate tests (2 × WAnTs).
- Measurements included lactate minimum intensity (LMI), blood lactate, venous pH, bicarbonate concentration ([HCO3]), oxygen uptake, and pulmonary ventilation.
Main Results:
- Lactate minimum intensity (LMI) was significantly higher following the 2 × WAnTs protocol compared to GXT and single WAnT.
- The 2 × WAnTs protocol resulted in lower venous pH and bicarbonate concentration ([HCO3]) but higher blood lactate levels compared to GXT and WAnT.
- Oxygen uptake and pulmonary ventilation at LMI did not differ significantly across the protocols.
Conclusions:
- The mode of hyperlactemia and acidosis induction significantly affects blood pH, lactate, and bicarbonate levels.
- These alterations in physiological markers consequently influence the determined lactate minimum intensity (LMI) and associated parameters.
- Exercise protocol selection is critical when assessing LMI and related physiological responses in athletes.
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