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[Lipoxygenase inhibitors and mast cell degranulation]
1Institut für Pharmakologische Forschung, VEB des Pharmazeutischen Kombinates Germed Dresden, Berlin-Friedrichsfelde, DDR.
Biomedica Biochimica Acta
|January 1, 1988
Summary
Lipoxygenase inhibitors and antioxidants prevent histamine release from rat mast cells. This suggests that oxygen radicals promote histamine secretion, and inhibitors may act via multiple mechanisms.
Area of Science:
- Biochemistry
- Immunology
- Pharmacology
Context:
- Mast cells are key players in allergic reactions, releasing histamine.
- Protaminesulfate stimulation of rat peritoneal mast cells (pRMZ) triggers histamine release.
- Lipoxygenase (LOX) pathway and oxidative stress are implicated in mast cell degranulation.
Purpose:
- To investigate the role of lipoxygenase inhibitors and antioxidants in modulating histamine release from stimulated rat mast cells.
- To elucidate the mechanisms by which these agents affect mast cell degranulation.
Summary:
- Lipoxygenase inhibitors block histamine release from protaminesulfate-stimulated rat mast cells in vitro.
- The inhibitory activity of agents against mast cell degranulation did not correlate with their direct lipoxygenase inhibitory activity.
- Antioxidants/radical scavengers also inhibited degranulation, suggesting a role for activated oxygen radicals in the process.
- The study proposes that lipoxygenase inhibitors with radical scavenging properties act through two mechanisms: reducing pro-inflammatory 5-LOX products and mitigating oxidative damage.
Impact:
- Provides evidence for the involvement of activated oxygen radicals in mast cell degranulation.
- Suggests a dual mechanism of action for lipoxygenase inhibitors in modulating allergic responses.
- Highlights the potential of combined lipoxygenase inhibition and antioxidant strategies for managing conditions involving mast cell activation.