Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

51.9K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
51.9K
PCR01:32

PCR

193.0K
Overview
193.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harmonization of measurement units: mission impossible or ethical imperative?

Clinical chemistry and laboratory medicine·2026
Same author

A Beginner Step-by-Step Guide for Gene Expression Analysis Using QIAcuity Digital PCR.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

When Two-Fold Is Not Enough: Quantifying Uncertainty in Low-Copy qPCR.

International journal of molecular sciences·2025
Same author

From crisis to routine - Standardization of SARS-CoV-2 genome detection by enhanced EQA schemes in a scientific pandemic network.

International journal of medical microbiology : IJMM·2025
Same author

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines.

Clinical chemistry·2025
Same author

Digital PCR-Based Gene Expression Analysis Using a Highly Multiplexed Assay with Universal Detection Probes to Study Induced Pluripotent Stem Cell Differentiation into Cranial Neural Crest Cells.

Methods in molecular biology (Clifton, N.J.)·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

14.9K

Minimum information necessary for quantitative real-time PCR experiments.

Gemma Johnson1, Afif Abdel Nour, Tania Nolan

  • 1Blizard Institute of Cellular and Molecular Science, Queen Mary University, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 18, 2014
PubMed
Summary
This summary is machine-generated.

The Minimum Information for Publication of Quantitative Real-Time PCR (qPCR) guidelines improve assay design and reporting. Adherence is crucial to prevent inaccurate scientific data and ensure reliable research findings.

More Related Videos

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

6.1K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

35.3K

Related Experiment Videos

Last Updated: May 1, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

14.9K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

6.1K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

35.3K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Scientific Publishing

Background:

  • Quantitative real-time PCR (qPCR) is a widely used technique.
  • Inconsistent and inaccurate data arise from poor assay design and inadequate reporting.
  • Lack of transparency in qPCR protocols hinders data validation and reproducibility.

Purpose of the Study:

  • To introduce the Minimum Information for Publication of Quantitative Real-Time PCR (MIQE) guidelines.
  • To provide a standard for qPCR assay design and reporting.
  • To address the issue of unreliable data in the scientific literature.

Main Methods:

  • Development and dissemination of the MIQE guidelines.
  • Encouraging comprehensive reporting of qPCR protocols.
  • Highlighting the importance of transparency in experimental details.

Main Results:

  • The MIQE guidelines have gained wide acceptance, evidenced by over 2,200 citations.
  • The guidelines aim to improve the quality and reliability of published qPCR data.
  • A significant body of literature may contain inaccurate data due to non-adherence to reporting standards.

Conclusions:

  • The MIQE guidelines are essential for robust qPCR research.
  • Widespread adoption of MIQE is necessary to improve the integrity of the scientific literature.
  • Correcting existing inconsistencies in life sciences research requires addressing data quality issues stemming from qPCR protocols.