Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assessing the Metabolic Activity of Whole Regenerating Zebrafish Ventricles Ex Vivo Using an Extracellular Flux Assay.

Journal of visualized experiments : JoVE·2026

Same author

Expanded iOn switch toolkit enables flexible clonal labeling and dynamic imaging in model and non-model animals.

Communications biology·2026

Same author

Foxg1 Defines Ventrolateral Zonal Identity by Sustaining Neurogenic Progenitor Potential in the Olfactory Epithelium.

Genes to cells : devoted to molecular & cellular mechanisms·2026

Same author

Distinct contributions of calmodulin-encoding genes to the migration and development of neocortical layer 4 neurons.

Biochemical and biophysical research communications·2025

Same author

Endothelial Slit2 guides the Robo1-positive sympathetic innervation during heart development.

eLife·2025

Same author

Lineage Analysis of Pallial Subdivision-Derived Cells in the Developing Chick Pallium.

Development, growth & differentiation·2025

Search research articles

Related Experiment Video

Updated: May 1, 2026

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Published on: May 5, 2022

Robo1 modulates proliferation and neurogenesis in the developing neocortex.

Mason L Yeh¹, Yuko Gonda, Mathilda T M Mommersteeg

¹Department of Cell and Developmental Biology, University College London, London, United Kingdom WC1E 6DE, Laboratory for Neocortical Development, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan, Institut Jacques-Monod, Université Paris Diderot/CNRS, 75201 Paris, France, and Institut de la Vision, INSERM UMRS 968, F75012 Paris, France.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience

|April 18, 2014

Summary

The Roundabout (Robo)1 receptor regulates pyramidal neuron proliferation in the developing mammalian neocortex. Robo1 deficiency leads to increased progenitor cells and pyramidal neurons, which are later lost postnatally.

Keywords:

Robo corticogenesis progenitors proliferation

More Related Videos

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Related Experiment Videos

Last Updated: May 1, 2026

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Published on: May 5, 2022

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Mammalian neocortex development involves precise neuron production and layering.
Chemotropic factors guide neuronal development, with Roundabout (Robo) receptors and Slit ligands known to influence interneuron migration.
The role of Robo and Slit in pyramidal neuron development is less understood.

Purpose of the Study:

To investigate the function of Robo1 and Slit genes in mammalian neocortex development, specifically focusing on pyramidal neuron generation.
To determine the impact of Robo1 and Slit gene deficiencies on progenitor cell proliferation and neuronal populations.

Main Methods:

Gene expression analysis of Robo1, Slit1, and Slit2 in the developing forebrain.
Phenotypic analysis of knockout mouse models (Robo1-/-, Slit1-/-, Slit1-/-/Slit2-/-).
Utilized layer-specific markers to assess pyramidal neuron populations at different developmental stages.
Employed in utero electroporation with short hairpin RNA (shRNA) to target progenitor cells.
Performed dissociated cortical cell cultures to confirm findings.

Main Results:

Robo1 and Slit genes are expressed in cells lining the telencephalic ventricles.
Robo1-/-, Slit1-/-, and Slit1-/-/Slit2-/- mice showed increased progenitor cells at embryonic days 12.5 and 14.5.
Robo1-/- mice exhibited significantly increased populations of both early- and late-born pyramidal neurons at embryonic day 18.5.
The increase in pyramidal neurons resulted from prolonged progenitor proliferation, not altered cell cycle dynamics.
Excess prenatal pyramidal neurons underwent postnatal cell death.

Conclusions:

Robo1 plays a novel role in regulating the proliferation and generation of pyramidal neurons in the developing neocortex.
Disruption of the Robo1/Slit signaling pathway affects progenitor cell behavior and subsequent neuronal output.
Understanding these mechanisms is crucial for comprehending neocortical cytoarchitecture and development.