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Updated: Aug 6, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification
Ramiz I Nathani1, Paul Moody1, Vijay Chudasama1
1Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
Abstract:
Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.

