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Related Concept Videos

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Hematopoiesis01:21

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Regulation of Hematopoietic Stem Cells01:01

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Commitment is the  process whereby stem cells:
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Overview of Hematopoiesis01:20

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Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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Correlating histone modification patterns with gene expression data during hematopoiesis.

Gangqing Hu1, Keji Zhao

  • 1Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA, gangqing.hu@nih.gov.

Methods in Molecular Biology (Clifton, N.J.)
|April 19, 2014
PubMed
Summary
This summary is machine-generated.

Hematopoietic stem cells (HSCs) differentiate into blood cells, regulated by transcription factors and epigenetic modifications. This review covers bioinformatics analysis of ChIP-Seq and RNA-Seq data for studying these epigenetic mechanisms in hematopoiesis.

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Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry
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Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry
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Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry

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Area of Science:

  • Hematopoiesis research
  • Epigenetics
  • Bioinformatics

Background:

  • Mammalian hematopoietic stem cells (HSCs) are a key model for studying cell differentiation.
  • Transcription factors (TFs) are known regulators of HSC differentiation.
  • Emerging evidence highlights the role of chromatin structure and epigenetic modifications in gene regulation during hematopoiesis.

Purpose of the Study:

  • To review bioinformatics approaches for analyzing ChIP-Seq and RNA-Seq data.
  • To provide a practical guide to basic data analysis methods for epigenetic studies in hematopoiesis.

Main Methods:

  • Review of bioinformatics tools and pipelines for ChIP-Seq data analysis.
  • Overview of RNA-Seq data analysis techniques.
  • Integration of ChIP-Seq and RNA-Seq data for epigenetic profiling.

Main Results:

  • Histone modifications, identified through genome-wide studies, are significantly linked to gene expression patterns in hematopoiesis.
  • Bioinformatics analysis of sequencing data is crucial for understanding the epigenetic landscape of blood cell development.

Conclusions:

  • Epigenetic modifications play a critical role in regulating gene expression during HSC differentiation.
  • Effective bioinformatics analysis of ChIP-Seq and RNA-Seq data is essential for advancing our understanding of hematopoiesis.