Simultaneous determination of ascorbic acid, dopamine and uric acid based on tryptophan functionalized graphene

Area of Science:

• Materials Science
• Electrochemistry
• Nanotechnology

Background:

• Graphene (GR) is a promising material due to its excellent conductivity.
• Functionalization of graphene can enhance its properties for specific applications.
• Developing efficient methods for simultaneous detection of biomolecules is crucial.

Purpose of the Study:

• To synthesize a novel tryptophan-functionalized graphene nanocomposite (Trp-GR).
• To investigate the electrochemical properties of Trp-GR for sensing applications.
• To develop a method for simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA).

Main Methods:

• Synthesis of Trp-GR via ultrasonic method utilizing π-π conjugation.
• Characterization of Trp-GR using SEM, TEM, and Raman spectroscopy.
• Electrochemical analysis using cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

Main Results:

• Trp-GR showed improved dispersivity in water and conductivity compared to pure GR.
• Successful simultaneous detection of AA, DA, and UA with well-separated oxidation peaks.
• Achieved low detection limits for AA (10.09 μM), DA (0.29 μM), and UA (1.24 μM) using DPV.
• Demonstrated potential for real sample analysis.

Conclusions:

• The synthesized Trp-GR is a highly effective material for electrochemical sensing.
• The Trp-GR modified electrode allows for sensitive and simultaneous detection of AA, DA, and UA.
• This work offers a new platform for developing advanced electrochemical sensors.