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Quantification of Atherosclerosis in Mice
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Group 1B phospholipase A₂ inactivation suppresses atherosclerosis and metabolic diseases in LDL receptor-deficient

Norris I Hollie1, Eddy S Konaniah1, Colleen Goodin1

  • 1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45237, USA.

Atherosclerosis
|April 22, 2014
PubMed
Abstract

Insights

Inactivating group 1B phospholipase A2 (Pla2g1b) prevents diet-induced obesity, diabetes, and atherosclerosis in mice. This suggests targeting Pla2g1b may reduce these conditions in humans.

Area of Science:

  • Biochemistry
  • Metabolic Diseases
  • Cardiovascular Research

Background:

  • Group 1B phospholipase A2 (Pla2g1b) inactivation is known to mitigate diet-induced metabolic dysfunction in C57BL/6 mice.
  • The impact of Pla2g1b inactivation on atherosclerosis has not been previously investigated.

Purpose of the Study:

  • To evaluate the efficacy of Pla2g1b inactivation in preventing atherosclerosis in a preclinical model.
  • To assess the role of Pla2g1b in diet-induced obesity, hyperglycemia, and hyperlipidemia.

Main Methods:

  • Utilized LDL receptor-deficient (Ldlr(-/-)) mice, exhibiting human-like hyperlipidemia.
  • Administered a hypercaloric diet (58.5% fat, 25% sucrose) for 10 weeks.
  • Compared atherosclerosis development and metabolic markers between Pla2g1b(+/+)Ldlr(-/-) and Pla2g1b(-/-)Ldlr(-/-) mice.

Main Results:

  • Pla2g1b(-/-)Ldlr(-/-) mice were resistant to diet-induced body weight gain.
  • These mice showed significantly lower fasting glucose, insulin, and plasma lipid levels.
  • Atherosclerotic lesions in the aortic roots were reduced seven-fold in Pla2g1b(-/-)Ldlr(-/-) mice.

Conclusions:

  • Pla2g1b inactivation effectively suppresses diet-induced obesity, diabetes, and atherosclerosis in Ldlr(-/-) mice.
  • Pharmacological inhibition of Pla2g1b presents a potential therapeutic strategy for reducing obesity and associated risks in humans.
Keywords:
AtherosclerosisGlucose toleranceLipid and lipoprotein metabolismPhospholipase

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