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Updated: May 1, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
Published on: March 19, 2010
An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions
Emmanuelle Tchekanda1, Durga Sivanesan1, Stephen W Michnick1
1Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.
Abstract:
We report a protein-fragment complementation assay (PCA) based on the engineered Deinococcus radiodurans infrared fluorescent protein IFP1.4. Unlike previous fluorescent protein PCAs, the IFP PCA is reversible, allowing analysis of spatiotemporal dynamics of hormone-induced signaling complexes in living yeast and mammalian cells at nanometer resolution. The inherently low background of infrared fluorescence permitted detection of subcellular reorganization of a signaling complex expressed at low abundance.
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