Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Health professions education in a constrained financing era: implications of the One Big Beautiful Bill Act.

Academic medicine : journal of the Association of American Medical Colleges·2026

Same author

MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryos.

Nature communications·2026

Same author

Peripheral Nerve Injuries in Patients Undergoing Congenital Cardiac Surgery and Cardiac Catheterization.

Journal of cardiothoracic and vascular anesthesia·2026

Same author

Click-to-Release Reactions for Tertiary Amines and Pyridines.

Journal of the American Chemical Society·2026

Same author

Aryl azopyrroles as visible light photoswitchable TRPA1 ligands.

Chemical science·2025

Same author

Combined Inductive and Dispersion Effects Enhance Bioorthogonal Reactivity of Tetrazines Toward Isonitriles.

Angewandte Chemie (International ed. in English)·2025

Search research articles

Related Experiment Video

Updated: May 1, 2026

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Published on: March 31, 2015

Methods for targeted mutagenesis in zebrafish using TALENs.

Woong Y Hwang¹, Randall T Peterson², Jing-Ruey Joanna Yeh¹

¹Cardiovascular Research Center, Massachusetts General Hospital, 149 13th street, Room 4201, Charlestown, MA 02129, USA; Department of Medicine, Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.

Methods (San Diego, Calif.)

|April 22, 2014

Summary

This summary is machine-generated.

Researchers can now easily create targeted mutations in zebrafish using transcription activator-like effector (TALE) nucleases (TALENs). These methods offer high success rates for generating mutations in genes of interest.

Keywords:

Gene disruption Gene targeting Genome engineering Knockout Site-specific nuclease Transcription activator-like effector nuclease Zebrafish

More Related Videos

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Published on: May 13, 2016

Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

Published on: April 22, 2019

Related Experiment Videos

Last Updated: May 1, 2026

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Published on: March 31, 2015

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Published on: May 13, 2016

Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

Published on: April 22, 2019

Area of Science:

Molecular Biology
Genetics
Zebrafish Model Organisms

Background:

Transcription activator-like effector (TALE) nucleases (TALENs) are engineered restriction enzymes.
TALENs enable targeted DNA modification by binding to specific DNA sequences.
Zebrafish are a key model organism for genetic studies.

Purpose of the Study:

To describe methods for constructing TALEN vectors for zebrafish.
To present techniques for detecting TALEN-induced mutations in zebrafish.
To facilitate targeted mutagenesis in zebrafish research.

Main Methods:

Construction of TALEN vectors utilizing TALE repeats for DNA-binding specificity.
Application of TALENs to induce mutations in zebrafish at desired genomic loci.
Development of protocols for detecting induced mutations.

Main Results:

High success rates achieved in constructing functional TALEN vectors.
Demonstrated high mutation efficiencies in zebrafish using the described methods.
Successful detection of TALEN-induced mutations at various genomic locations.

Conclusions:

The described methods enable efficient generation of targeted mutations in zebrafish.
These protocols empower researchers to study gene function through mutagenesis.
TALEN technology provides a versatile tool for zebrafish genetic research.