Effects of pathogen reduction systems on platelet microRNAs, mRNAs, activation, and function

Area of Science:

• Hematology
• Transfusion Medicine
• Molecular Biology

Background:

• Pathogen reduction (PR) systems aim to prevent transfusion-transmitted infections.
• Some PR systems have been linked to increased bleeding risk in clinical studies.
• The impact of PR on platelet nucleic acid content and function requires further investigation.

Purpose of the Study:

• To investigate the effects of two PR systems (Intercept and Mirasol) on microRNA and mRNA levels in stored platelets.
• To assess the impact of PR on platelet activation and aggregation function.
• To determine if PR affects platelet nucleic acid synthesis or induces cross-linking.

Main Methods:

• Platelets stored in plasma were treated with Intercept (amotosalen + UVA light) or Mirasol (riboflavin + UVB light).
• MicroRNA and mRNA levels were quantified using molecular assays.
• Platelet activation and aggregation responses to ADP were measured.
• Platelet microRNA synthesis and RNA cross-linking were evaluated.

Main Results:

• Intercept treatment significantly reduced levels of 6/11 microRNAs and 2/3 anti-apoptotic mRNAs (Bcl-xl, Clusterin) compared to controls.
• Mirasol treatment did not yield similar reductions in nucleic acid levels.
• Intercept-induced reduction in microRNAs correlated with increased platelet activation and impaired aggregation response to ADP.
• PR did not affect platelet microRNA synthesis or induce cross-linking of endogenous platelet RNA.

Conclusions:

• Intercept, but not Mirasol, significantly reduces microRNA and mRNA levels in stored platelets.
• Intercept treatment may induce platelet activation, leading to nucleic acid release.
• The clinical significance of Intercept-induced reduction in platelet nucleic acids warrants further study.