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Updated: May 1, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
Published on: March 14, 2018
Involvement of satellite I noncoding RNA in regulation of chromosome segregation
Takashi Ideue1, Yukiko Cho, Kanako Nishimura
1Department of Biological Sciences, Graduate School of Science Technology, Kumamoto University, Kumamoto, 860-8555, Japan.
Abstract:
Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape-shape phenotype. Co-immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Satellite I RNA was also shown to associate with INCENP, another component of the CPC. In addition, depletion of satellite I RNA resulted in up-regulation of kinase activity of Aurora B and delocalization of the CPC from the centromere region. These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase.
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