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Complete nucleotide sequence of two multidrug-resistant IncR plasmids from Klebsiella pneumoniae

Fabrice Compain1, Lionel Frangeul2, Laurence Drieux3

  • 1Laboratoire de Bactériologie, Faculté de Médecine, Université Pierre et Marie Curie, Paris, France.

Antimicrobial Agents and Chemotherapy
|April 23, 2014
PubMed

Insights

Two IncR plasmids carrying multiple antibiotic resistance genes, including those for beta-lactams, aminoglycosides, and fluoroquinolones, were sequenced. IncR replicons are increasingly important carriers of antimicrobial resistance determinants.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • The emergence of multidrug-resistant bacteria poses a significant global health threat.
  • Plasmids are key mobile genetic elements that facilitate the spread of antibiotic resistance genes.
  • IncR replicons are a class of plasmids increasingly identified in clinical settings.

Purpose of the Study:

  • To determine the complete nucleotide sequence of two IncR replicons.
  • To identify the antibiotic resistance genes and genetic elements harbored by these plasmids.
  • To understand the role of IncR replicons in carrying and disseminating antimicrobial resistance.

Main Methods:

  • Whole-genome sequencing of two IncR plasmids.
  • Bioinformatic analysis to identify replication and stability genes.
  • Identification and characterization of transposable elements and antibiotic resistance genes.

Main Results:

  • Complete nucleotide sequences of two IncR replicons were obtained.
  • Plasmids share similar backbones, including replication and stability systems.
  • A variety of transposable elements carrying beta-lactam, aminoglycoside, and fluoroquinolone resistance genes (blaDHA-1, blaSHV-12, aphA1, strA, strB, qnrB4, aac6'-1b-cr) were identified.

Conclusions:

  • IncR replicons possess genetic features conducive to carrying diverse antibiotic resistance genes.
  • The identified resistance genes confer resistance to critical antibiotic classes.
  • This study highlights the growing clinical significance of IncR replicons as vectors for antimicrobial resistance.

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