Synthesis of nano-hydroxyapatite via microbial method and its characterization

Area of Science:

• Materials Science
• Biotechnology
• Nanotechnology

Background:

• Hydroxyapatite is a key biomaterial for bone regeneration and dental applications.
• Conventional synthesis methods often require high temperatures and pressures.
• Developing sustainable and efficient synthesis routes is crucial for biomaterials.

Purpose of the Study:

• To synthesize hydroxyapatite nanoparticles using a microbial method at ambient conditions.
• To characterize the synthesized hydroxyapatite nanoparticles.
• To compare the microbial synthesis with traditional chemical precipitation methods.

Main Methods:

• Microbial synthesis using calcium chloride and a specific substrate.
• Characterization via X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and thermogravimetric analysis (TGA).

Main Results:

• Successful synthesis of hydroxyapatite nanoparticles at ambient temperature and pressure.
• Characterization confirmed the phase composition, functional groups, and surface morphology.
• Microbial synthesis yielded results comparable to chemical precipitation.
• The hydroxyapatite powder exhibited nanometer-grade size and sphere-like morphology.

Conclusions:

• Microbial synthesis offers a viable, ambient-condition alternative for producing hydroxyapatite nanoparticles.
• The synthesized nanoparticles possess characteristics suitable for biomaterial applications.
• This approach presents a more sustainable and energy-efficient route for hydroxyapatite production.