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Updated: Apr 30, 2026
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Published on: August 3, 2011
A superior strategy for single-cell mutational screening via multiplex-targeted QPCR using the BioMark HD
1Department of Surgical Oncology, the 1st Affiliated Hospital, School of Medicine, Zhejiang University, 79, Qingchun Road, Hangzhou, Zhejiang, China.
This study introduces a new method for analyzing cancer cell genomics. It enables detailed single-cell analysis of mutations, gene fusions, and copy number alterations for personalized cancer medicine.
Area of Science:
- Genomics
- Cancer Biology
- Molecular Diagnostics
Background:
- Cancer's complexity and heterogeneity necessitate personalized medicine approaches.
- Current genomic technologies for cancer have limitations including error rates, low throughput, high cost, and labor intensity.
- Dissecting cancer's clonal architecture via genomics is crucial for advancing personalized medicine.
Purpose of the Study:
- To develop a superior strategy for comprehensive interrogation of cancer cell genomics.
- To enable detailed analysis of single cancer cells for personalized medicine.
Main Methods:
- Multiplex-targeted DNA amplification from flow-sorted single cells.
- High-throughput quantitative PCR using the BioMark HD microfluidic platform.
- Simultaneous detection of chimeric fusion genes, copy number alterations, and single-nucleotide variants.
Main Results:
- Achieved a successful analysis rate of approximately 75%.
- Demonstrated a highly efficient single-cell sorting rate of 96-98%.
- Enabled high-throughput analysis of 200-300 leukemic cells simultaneously.
Conclusions:
- The developed platform offers a comprehensive approach to interrogate complex cancer cell genomics.
- This method advances personalized medicine by providing detailed single-cell genomic information.
- The platform's efficiency and throughput address limitations of current genomic technologies.
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